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Notably, the entire salvage pathway for PC synthesis has been modeled in this manner by reconstitution of four semipurified enzymes (G3P-AT, LPA-AT, PAP, and CDPC-PT) in liposomes composed of soybean PC and sodium deoxycholate

Optical density measurements were taken at 450 nm using an ELISA plate reader (BioTek Instruments, Highland Park, IL), with a reference wavelength set between 620 and 630 nm