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Corona, J

Drink plenty of fluids such as water or electrolyte drinks

It has been proven that many abnormal physiological and metabolic processes in organisms produce biological oxidants such as superoxide radicals (O 2 ), hydroxyl radicals (OH), and hydrogen peroxide (H 2 O 2 ), which could endow several different biological targets with reactivity and instability, such as DNA, proteins, and membrane lipids [5]

Over the years, methods have been refined into the most commonly used protocol today, which uses an acid/ethanol precipitation (to disrupt the IGF-1/IGFBP complex) followed by the addition of insulin-like growth factor-2 (IGF-2) to prevent the reformation of the IGF/IGFBP complex

This is what is missing

PMID: 39740402 Nefrologia2023 Sep 6