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Fragments HS-His3MX6-pTEF1-HS (described earlier) and HS-KanMX-pGSH1-HS were amplified and used to replace pGSH1 with pTEF1 and pGAP1 with pGSH1 in both of the BY4741 and BY4742 strains (giving genotype pgsh1 D:: His3MX6-pTEF1

Seong J, Kang JY, Sun JS, Kim KW (2019) Hypothalamic inflammation and obesity: a mechanistic review

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(PubMed) Desmond MA, Sobiecki JG, Jaworski M, et al