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LLO-mediated mitochondrial damage promotes mtROS accumulation, a potent NLRP3 activator, leading to IL-1 secretion and pyroptosis ( Brucella abortus deploys effector proteins such as BtpA, which interfere with mitochondrial metabolism and immune signaling, thereby suppressing proinflammatory cytokine production (Qin et al., 2024)

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