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A recent study combining single-nucleus sequencing and spatial transcriptomics pinpointed the human hypothalamic GLP-1R-LepR co-expressing cluster, which strongly correlated with the mouse hypothalamic cluster that exhibits the second highest expression of GLP-1R in the hypothalamus [76]

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For these assays, increased levels of cAMP were measured as an increase of the 485/535 nm FRET ratio serving as a readout for binding of cAMP to the H188 biosensor that is based on the exchange protein activated by cAMP 15

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AAPS PharmSciTech 21:112