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Reactivation procedure for oxidized e-GST Different hemolyzed blood samples (total blood, isolated erythrocytes or serum) and purified BSA approximately at the same concentration present in hemolyzed blood (23 M), were all incubated for 60 min at 37 C in a dry block thermostat with 1 mM final concentration of DTT and 0.01 M potassium phosphate buffer (pH 8.0)

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Mass spectrometry experiments were carried out using an Orbitrap Fusion LUMOS instrument (Thermo) paired with a Vanquish Neo ultra-performance liquid chromatography (UPLC) system (Thermo)