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doi: 10.1016/S2213-8587(19)30086-5 55 PykeC.HellerR

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Insoluble material was removed by centrifugation at 30,000 g for 30 min and the solubilised complex was immobilised by batch binding to M1 anti-FLAG affinity resin in the presence of 5 mM CaCl 2

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