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Bands was sequentially incubated with 10 mM DTT ammonium bicarbonate solution (for 30 min at 56 C), for protein denaturation, 55 mM IAA ammonium bicarbonate solution (for 20 min, at room temperature (RT), protected from light) for protein acetylation and with 100 mM ammonium bicarbonate/acetonitrile (AMBIC/ACN 1:1 v/v) (for 30 min at RT) for the destaining process

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influenzae , was also found to induce MUC5AC gene expression in human lung epithelial cells and in mice trachea treated with P6 (Chen et al., 2004)