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After 48 h, 160 l of expression medium (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-galactose (Panreac, A1131), 67 mM potassium phosphate monobasic buffer pH 6.0 (Thermo Scientific, 424200250), 25 g l 1 ethanol (Scharlau, ET00062500), 2 mM copper sulfate (Merck, C7631) and 25 g ml 1 chloramphenicol (Merck, C0378)) was added to each well
Martinotti G, Pettorruso M, Montemitro C, Spagnolo PA, Acuti Martellucci C, Di Carlo F, et al
Look for the active ingredient: docusate sodium
For oral melanoma, which is a rare but aggressive malignancy, early recognition and treatment are crucial
Route selection determines not only clearance kinetics but also peak concentration, bioavailability, and CNS penetration efficiency