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Assessment of 8-hydroxy-guanosine (oh8G) as an indicator of oxidative DNA damage has displayed that ROS-induced DNA oxidation during the freezing procedures can be decreased after adding L-carnitine to the cryomedium (81)
Mol Cancer Res (2018) 16:63442
Do not store it in the door compartment where temperature fluctuates every time the door opens
Before each assay appropriate amount of aliquots were thawed, suspended (1 10 5 cells/ml) in assay medium (DMEM w/o phenol red, 10 mM Hepes, 2% ovalbumin and 0.2% Pluronic F-68) and then transferred into a 96 well plate (5 10 3 cells/50 l) followed by addition of ligand and/or Fab 3F52 diluted in assay medium (50 l/well)
