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It consisted of serum-free, high-glucose Dulbeccos Modified Eagle Medium (DMEM) (Gibco, Grand Island, NY) with 1% Penicillin-Streptomycin by volume, 1 mg/mL collagenase IV (Gibco), and 0.2 mg/mL DNase (Sigma-Aldrich)

They also consumed less alcohol

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It can offer access to treatment for many more of the estimated 200 million Americans who are overweight or obese

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They observed no difference in this parameter between the presynaptic boutons even under high-intensity stimulation of neurotransmitter release (600AP) [58]