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Hoffman AR, Kuntze JE, Baptista J, et al

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Targeted Metabolomics on Plasma Metabolite extraction Plasma (20L from 29 female and male samples) was transferred to an individual 1.5 mL microcentrifuge tube and incubated with 400L 80 C 80:20 Methanol (MeOH): H2O extraction solvent on dry ice for 5 minutes post-vortexing

Fertil Steril 106(3):731738

By avoiding traditional pharmacological treatments that may produce significant side effects, parents try to alleviate behavioral problems and associated comorbid conditions using alternative treatments

These come under the neurological symptoms of vitamin b12 deficiency