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The microorganisms were cultured and counted using the dilution plate culture counting method

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Specifically for the tadpoles of the Green Frog, they reacted with spatial anti-predator behavior (distancing themselves from the chemical cues), when only exposed to both Anax kairomones and conspecific alarm cues together

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However, GIP immunoreactivity was identified in the adult rat brain using a specific monoclonal antibody against GIP (136), suggesting that while GIP is not expressed in the brain, peripheral GIP can reach the deep brain