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Upon tissue disruption glucosinolates are enzymatically degraded by myrosinase and may yield a variety of biologically active products such as isothiocyanates, thiocyanates, nitriles and oxazolidine-2-thiones

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The resulting cDNA was preamplified with 12 thermal cycles using the Fluidigm Preamp Master Mix and Delta Gene Assays according to manufacturers protocol