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Monocyte separation In a 15 ml centrifuge tube carefully layer 6 ml of LRP over 3 ml of the density barrier (avoid mixing at the interface)

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Peptides (1996) 17:11924

Major metabolites found were trimethylamine N-oxide, primarily in urine (8% to 49% of the administered dose) and [ 3H]--butyrobetaine, primarily in feces (0.44% to 45% of the administered dose)