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after reduction and alkylation using DTT and IAA, the proteins were centrifuged on a 30 kDa cutoff filter device (Sartorius), washed twice with UA buffer (8 M urea in 0.1 M Tris/HCl pH 8.5) and twice with 50 mM ammoniumbicarbonate

Over this period, the annual number of measurements increased more than 12-fold, rising from 2 million in 2016 to 25 million in 2023

(2001) identified specific genes that are dependent on H 2 O 2 for either expression or repression

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