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On the second day, all animal groups were administered different compounds via oral gavage after 1 hour of fasting, followed, the next hour, by the choline chloride load

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GSH = glutathione

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At the experimental endpoint, the cells were fixed with 10% trichloroacetic acid (Sigma-Aldrich, T6399-500G) and stained with 0.05% SRB for 1 h and then washed repeatedly with 1% acetic acid

Another form of vesicular transport pinocytosis, which is responsible for the transport of fluids across cells, is largely lacking in the BBB [25]