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Western blot analysis Request a detailed protocol Tissues were homogenized in 0.5 ml ice-cold T-PER tissue protein extraction buffer (Thermo Scientific, Rockford, IL) with protease and phosphatase inhibitors (Sigma)

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Among the 10 PDPs offered in most or all PDP regions, most charge $0 for preferred generics but only 1 PDP charges flat copayments for preferred brands and all charge coinsurance for non-preferred drugs Part D enrollees in 8 of the 10 national or near-national PDPs face a median copayment of $0 for preferred generics, while median copays for drugs on the standard generic tier range from $0 to $10 (Figure 9)

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