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Each freeze-thaw cycle stresses the peptide in solution and can cause aggregation, the process by which peptide molecules clump together and lose biological activity

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Structural deficits include EC degeneration, detachment of astrocytic endfeet, and pericyte degeneration that cause instability of the vascular architecture, as well as reduced expression of TJ proteins claudin-5 and occludin (Winkler et al., 2011