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This latter process depends on glutamate released from dendritic cells, but also on extracellular glutamate concentrations, suggesting that this process could be influenced by dietary glutamate (179)

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Briefly, plasmids containing the gene of interest were constructed using the Gibson assembly method [92] and microinjected at 50 ng/l along with transposase and co-injection markers to target the ttTi5605 or cxTi10816 sites