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Major malformations risk following early pregnancy exposure to metformin: a systematic review and meta-analysis

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(download DOCX Additional file 1 contains supplemental Materials and Methods, supplemental table 1 with the list of used probes and primers for gene expression analysis and supplemental table 2 with the list of used antibodies for protein expression analysis

DNA sample were digested to nucleosides by incubating the denatured DNA with 3 L sodium acetate (20 mM, pH 5.2) and 2 L of nuclease P1 (6.25 U/L) for 2 h at 37 C

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Some patients get excellent results with natural protocols or benefit from combining medication with bioidentical hormone therapy to address perimenopausal weight gain