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It mimics the hormone GLP-1, increasing insulin secretion, slowing gastric emptying, and reducing appetite

Effect of quercetin and umbelliferone on the transcript level of Penicillium expansum genes involved in patulin biosynthesis

Importantly, tirzepatide shows preferential potency at GIPR relative to GLP-1R, which is one reason its metabolic effect profile differs from GLP-1 mono-agonists such as semaglutide

Endocrinology 155 , 45914601 (2014)

For immunostaining, cells were permeabilized for 1 min using ice-cold PBS supplemented with 0.1% Triton X-100, blocked for 30 min with PBS supplemented with 1% BSA, incubated with the primary antibody in blocking buffer for 2 h at room temperature (RT), washed and subsequently incubated with secondary antibodies and DAPI in blocking buffer for 2 h at RT

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