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Acute cerebral hypoxia caused by thrombosis during ischemia triggers inflammatory cascades, and immune activation can cause secondary damage within the penumbra of the ischemic infarct (Kamel and Iadecola, 2012)

Splitting the dose morning and evening may provide more consistent levels given the ~4 hour half-life

You can get cat-specific supplements such as Pet-tinic (US), Hemovet or Tonivit (EU), Thorne's B-Complex vet, etc

Sleep improvement is typically the earliest noticeable benefit

2017 Cell study used 5 mg/kg intraperitoneal injection three times per week in aged mice, with treatment periods of several weeks the three-times-weekly dosing suggests the peptide maintains sufficient functional concentrations for at least 48-72 hours per dose (PMID: 28340339)