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IGF-1 is a potent survival factor for endothelial cells, stimulating NO production and protecting against oxidative stress-induced apoptosis (126, 127)

You can also always consult your primary healthcare provider to discuss your concerns

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Tissues were homogenized using a Tenbroeck homogenizer in 50 mM K-phosphate buffer, pH 7.4, containing 0.25 M sucrose, 1 mM K-EDTA, and 0.1 mM dithiothreitol