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Query haplotypes classified with discordant shortest-distance and average-distance subtyping or with bootstrap scores below 70 were tagged as doubtful

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The catechol moieties of Tiron bind tightly to Fe + , resulting in a well-defined complex chemically represented as [Fe(C 6 H 4 Na 2 O 8 S 2 )] + , as illustrated in Scheme 1

For immunofluorescence examination, after deparaffinization and rehydration, antigen retrieval with target retrieval solution was performed, and the sections were blocked and incubated with the respective primary antibodies overnight at 4C