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Secondary antibodies were AlexaFluor-488 goat anti-mouse (Invitrogen A11001, lot 2513496), AlexaFluor-647 goat anti-rabbit (Invitrogen A32733, lot XG349344), AlexaFluor-488 goat anti-rabbit (Invitrogen A11008, lot 2420731) all diluted 1:500

Isothiocyanates react with certain regions on Keap1, eliminating Keap1s ability to target Nrf2 for degradation effectively serving the role of stressor

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The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)