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Biologic Exclusivity under the BPCIA, as noted, provides 12 years from the date of approval for reference products, plus a four-year data exclusivity period during which no biosimilar application can be submitted [29]

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On the one hand, Akkermansia muciniphila enhances the junction strength between intestinal epithelial cells by regulating the expression of tight junction proteins, such as ZO-1, Occludin, and Claudin-1, to attempt to repair the disrupted intestinal barrier (Liu et al., 2023)

Tomar 1 mL del extracto en un tubo de ensayo de 15 mL, aadir 9 mL de acetonitrilo, mezclar en vrtex durante 1 min, luego tomar una porcin en un tubo de centrfuga de 5 mL, centrifugar a 16000 r/min durante 5 min

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This is called the adaptation period