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Description

Individual serine doublet and triple AA phospho-site alanine mutations were introduced at the SNAP/FLAG-hGLP-1R-NanoLuc plasmid as described above for the SNAP/FLAG-hGLP-1R construct

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The IC 50 values representing inhibitor concentrations in which TyrBm activity was reduced by 50% were obtained from dose-response curves (Supplementary Fig

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This dose was chosen based on a previous dose-escalation study and subsequent work demonstrating its efficacy to drive weight loss in a short period of time 8

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