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CCK-8 assay HUVECs were evenly seeded into 96-well plates at a density of 1 10^4 cells per well, with a culture medium volume of 100 L per well

Poor diet: High consumption of processed foods, sugars, and caffeine can disrupt the balance of the HPA axis

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We also proposed that if the calculated percentile falls between two observed allowed amounts, the 10th percentile allowed amount is the next highest observed value