Fluorescence labeling of substrates for biochemical assays All substrates were fluorescently labeled by sortase A-mediated fusion 78 of fluorescein (FAM) or TAMRA-containing peptides to the proteins N-terminal glycine exposed upon cotranslational cleavage of initiator methionine (for GGNMNAT16His) or TEV-mediated removal of the GST tag (for GGSGSBRD2/BRD3/BRD4)
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