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When glutaminolysis is obstructed, both cystine starvation and erastin treatment fail to trigger ROS accumulation, lipid peroxidation, and subsequent ferroptosis (Gao et al., 2015b

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2014 Cafarchia et al

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Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32