After decalcification, samples are rinsed in PBS, then are dissected carefully to remove the residual bone from the tissue so that just the attachment of cochlear duct to the modiolar core is remaining
The measurement of paracellular permeability is usually quite straightforward since the movement of ions and charged organic molecules can be measured as an electrical current and the movement of neutral molecules can be measured by assaying their concentration in the bloodstream or urine or in a compartment equivalent to the subepithelial space in mounted or perfused tissue or in cultured cells grown on permeable substrates
Modifier 59 may be necessary if performed with other skin tests
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Dissected tissue is then permeabilized in 0.5% PBS-Triton (PBS-T) at room temperature for a minimum of 3 hours
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