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Circulating INSL-5 levels are estimated to be in the picomolar range (164, 172), which is several orders of magnitude lower than the EC 50 of INSL-5 on RXRP4 (166) and the supraphysiological concentrations used in the majority of insulin secretion experiments may have contributed to the conflicting results

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(PubMed) Ohno S, Ohno Y, Suzuki N, Soma G, Inoue M

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However, research on this specific protocol for sublingual formulations remains limited compared to injectable data