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Protein was eluted with 10 mM glutathione in 50 mM Tris, pH 8.0, 5% glycerol, and examined for purity by SDS-PAGE and Coomassie staining

Analysis by HPLC with fluorescence detection was performed as previously described 43,44

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If rapid perfusion of drug is needed to the tissue with low FCD, slow injection rate of around 100 L/min would be favorably better than the fast single-shot injection at high injection rate of around 6 mL / min

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Ghrelin receptor agonists