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Protein was eluted with elution buffer 1 (50 mM TrisHCl (pH 8.0), 10 mM MgCl 2 , 10% glycerol, 300 mM NaCl and 250 mM imidazole) and elution buffer 2 (50 mM TrisHCl (pH 8.0), 10 mM MgCl 2 , 10% glycerol, 300 mM NaCl and 400 mM imidazole)

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