Following this, the samples were sequentially incubated with primary and secondary antibodies, and nuclei were stained with 4',6-diamino-2-phenylindole (DAPI) (Beyotime Biotechnology, China)
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Clinical evidence supports sustained weight loss when interventions are adjusted
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Consider not telling (blinding) one parent, a therapist, or teacher about the treatment and asking for their evaluation of the child
Variability in outcome measurements may arise due to the absence of standardized evaluation tools and diagnostic criteria employed across different research studies