Some specialized medical diets like those used to treat certain metabolic disorders, including phenylketonuria, homocystinuria, and maple syrup urine disease, need to be supplemented with selenium to ensure optimal selenium status in patients (51)
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Membranes were pretreated with 5% non-fat milk/TBST (1 h) before overnight exposure at 4 C to antibodies targeting MAPKAPK2 (ab32567), HO-1 (ab189491), KEAP1 (ab119403), Nrf2 (ab137550), -H2AX (ab81299), PARP-1 (ab191217), CHK2 (ab207446), phosphorylated CHK2 (p-CHK2, ab59408), TYR (ab170905), PMEL (ab137078), MITF (ab12039), p-HSP27 (ab155987), HSP27 (ab12351), -actin (ab8226), and Histone H3 (ab1791) (all from Abcam, UK)
10.1038/166194a0 178 EndoS.SaitoY.WadaA
Laboratory Handling: Reconstitution, Solubility, and Stability This section describes laboratory handling of GHK-Cu as a reference standard