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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)

doi:10.1365/s10337-006-0125-2 Skibiski R (2011) Identification of photodegradation product of amisulpride by ultra-high-pressure liquid chromatography-DAD/ESI-quadrupole time-of-flight-mass spectrometry

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Furthermore, the specific inhibition of NNMT by 5-amino-1mq avoids interference with other metabolic enzymes, ensuring the specificity and reliability of experimental results