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The samples were run with a Bio-Rad CFX96 Real-time PCR system with a reverse transcription reaction for 10 min at 50 C, polymerase activation and DNA denaturation for 1 min at 95 C followed by 39 cycles of amplification (denaturation for 10 s at 95 C, annealing/extension for 15 s at 60 C) and a melt-curve analysis at 6595 C in 0.5 C increments

Regional analysis showed semaglutide treatment reduced astrogliosis in the hippocampus brain region compared to the untreated controls, while no statistically significant difference was detected in the thalamus and cerebellum

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These insights make it an important research tool in behavioral neuroscience

*Many people will need to start with just 10 minutes of walking each day and work-up

At 5mg/mL, a 0.25mg dose requires drawing only 5 units on the syringe