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However, aliquoting the reconstituted solution into single-use vials immediately after mixing allows you to freeze those aliquots at 20C for 612 months, with only one thaw event before use

However, there are dynamic scaffold-based 3D models used to study flow shear stress, such as polydimethylsiloxane (PDMS) only models, and others that combine the hydrogel cell culturing techniques with more complex microfluidic systems, namely PDMS-derived, PDMS-hydrogel hybrids, or pure hydrogel models [259]

doi:10.1146/annurev.nutr.19.1.217

And its a very comprehensive process that weve built over the last few years

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