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Burlington, MA) in PBS was used to prevent non-specific binding for one hour at R.T

Moreover, self-administration without professional guidance may lead to improper dosing, raising safety concerns

This consistency contributes to stable signal intensity and reproducible analytical results

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Bacterial cells exposed to acidic pH undergo substantial structural damage to their cell wall, and intracellular macromolecules such as DNA and proteins (Cotter and Hill, 2003)