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Physical activity: It can be as simple as walking

The membrane was blocked with 3% non-fat milk (BioRad, Hercules, CA, United States) at room temperature for 1.5 h, washed, and incubated with primary antibodies (Supplementary Table 3) at 4C overnight on a rotating shaker

Pituitary Pharmacology: Observing the feedback loops of the hypothalamic-pituitary axis in a controlled environment