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For lysates preparation ( 31 ), cultures were centrifuged (10,000 g, 15 minutes), washed twice with potassium phosphate buffer (100 mM, pH 7.0), resuspended in borate buffer (50 mM, pH=8.4), and broken by mechanical lysis with glass beads in a tissue lyser (Qiagen, Courtaboeuf, France) 10 minutes, 50 Hz

Drinking plenty of water and limiting carbonated beverages helps prevent gas buildup, while avoiding high-fat foods slows digestion even further and can amplify bloating sensations

Feng Q, Besevic J, Conroy M, Omiyale W, Woodward M, Lacey B et al (2024) Waist-to-height ratio and body fat percentage as risk factors for ischemic cardiovascular disease: a prospective cohort study from uk biobank

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EslerWPRudolphJClausTHTangWBarucciNBrownSEet al