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Following incubation, cells were washed three times with ice-cold PBS and lysed by addition of 50 L lysis buffer (PBS supplemented with 20 mM Tris-HCl, 1% Triton X-100, pH 7.4)

Visual inspection can catch gross problems such as discoloration or particulates, and the Instructions for Use direct checking that the expiration date has not passed

She eats enough protein

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