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From 'Laughing Gas' to 'Galaxy Gas': An Updated Review on the Neurological, Pathologic, and Psychiatric Sequelae of Nitrous Oxide Abuse

Protein expression was induced by addition of 200 M IPTG following a cultivation of additional 30 min (160 rpm, 37 C)

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The study participants included males and females in the ratio of 7:3, suggesting a male preponderance which was in contrast to the study by Pandey et al ., and Momin et al