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For child-specific supplementation requirements of other nutrients, check out my Dietitians Guide to Vegan Supplements for Kids

Avoid Repeated Freezing and Thawing: If you anticipate needing to store the reconstituted Klow blend for a very long time (beyond its refrigerated stability), consider aliquoting it into smaller, single-use portions after reconstitution and then freezing these aliquots

discard any unused portion

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Cells were then incubated with continuous shaking at 45C for 30 min, before plating on HC medium containing 2% (w/v) glucose as carbon source lacking histidine to ensure plasmid retention