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Safety track record: Metformin has been used for over 60 years with an excellent safety profile

Immunoblots Cells or tissues were lysed in buffer (150 mM NaCl, 10 mM Tris pH 7.6, 0.1% SDS and 5 mM EDTA) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, 78445)

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Factors such as your baseline weight, activity level, nutrition, and metabolism all play a role in your outcomes

In the future, it might be used for more intense therapies for cancer treatment and other conditions