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FDA staff's own briefing document, posted publicly ahead of the meeting as part of the docket, proposes that TB-500 (free base) and TB-500 acetate not be added to the list, citing incomplete physicochemical characterization, a lack of evidence of effectiveness for wound healing (including an in-vitro fibroblast study in which TB-500 reportedly did not induce wound healing), and insufficient nonclinical and clinical safety data

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We cloned the original sensor and the two computational designs into vectors containing a fragment of histone H2B for nuclear targeting in mammalian cells (Figure 1c)

doi: 10.1038/s41467-017-02375-6 73 ChengJYangHLGuCJLiuYKShaoJZhuRet al