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The results appear to be consistent throughout the investigations, as indicated by the minimal heterogeneity (I 2 = 0.0%) as shown in Figure 7

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When OD 600 reached 1520, cells were collected by centrifugation and resuspended with BMMY medium (replacing glycerol in BMGY with 0.5%(v/v) methanol) to OD 600 of 34 for protein expression

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