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After seeding onto 96-well microtiter plates (48,000 cells per well), the medium was replaced by HBSS followed by 30 min incubation at 37 C

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Clinical description According to the clinical symptoms, glutathione synthetase deficiency can be classified as mild, moderate or severe [19]: Mild glutathione synthetase deficiency These patients show mild hemolytic anemia as their only clinical symptom